总裁把她的乳尖都吸大了,国产亚洲精品久久久久久,日久精品不卡一区二区,再深点灬舒服灬太大了少妇

當(dāng)前位置:首頁  >  技術(shù)文章  >  DDIT3對(duì)Luminal A型乳腺癌的影響

DDIT3對(duì)Luminal A型乳腺癌的影響

更新時(shí)間:2024-12-28  |  點(diǎn)擊率:67

20237月,黑龍江省科學(xué)院先進(jìn)技術(shù)研究所;黑龍江省科學(xué)院先進(jìn)技術(shù)研究所(Institute of Advanced Technology, Heilongjiang Academy of SciencesInstitute of Advanced Technology, Heilongjiang Academy of Sciences) Guoqing Huang老師研究團(tuán)隊(duì)在《Research Square》上發(fā)表論文:

The effect of DDIT3 on luminal A type breast cancer"

 

DDIT3對(duì)Luminal A型乳腺癌的影響"

 

Abstract

Purpose: To analyze the phenotypic changes of breast cancer (BC) cell before and after DDIT3 knockdown/overexpression, and preliminarily explore the regulatory mechanism. Also, to analyze the relationship between DDIT3 and prognosis by combining with bioinformatics methods, which provide a basis for further research on DDIT3 targeted treatment of BC.

Methods: Loss- and gain-of-function studies, DDIT3 in MCF-7 (luminal A), and RNA-seq analysis were employed to investigate the functional impact of DDIT3 on BC cell proliferation and drug resistance. The relationship between DDIT3 and the prognosis of BC patients was systematically assessed using the tissue microarray technique along with qRT-PCR and publicly available data.

Results: Survival analysis showed that patients with lower DDIT3 expression in luminal A type BC or BC patient which were undergoing endocrine therapy had a poorer prognosis, and DDIT3 expression was associated with overall survival (OS) significant. Following the knockdown of DDIT3 in MCF-7 cells, the proliferation rate was significantly increased, and drug resistance ability was just reversed. On the contrary, overexpression of DDIT3 had a relative inhibitory effect on target cell proliferation. Notably, the inhibition of DDIT3 expression upregulated TP63 and downregulated PDGFR, with the results being exactly opposite after the overexpression of DDIT3.

Conclusion: These results have revealed that DDIT3 plays a critical role in luminal A type BC cell proliferation and TAM resistance, and it holds potential prognostic value in BC. Overall, DDIT3 may exert its functions in luminal A type BC by modulating the expression of TP63 and PDGFR.


摘要:

目的:分析乳腺癌(BC)細(xì)胞DDIT3敲低/過表達(dá)前后的表型變化,并初步探討其調(diào)控機(jī)制。結(jié)合生物信息學(xué)方法分析DDIT3與預(yù)后的關(guān)系,為進(jìn)一步研究DDIT3靶向治療BC提供依據(jù)。

方法:通過功能缺失和功能獲得研究、MCF-7 (luminal A)中的DDIT3RNA-seq分析來研究DDIT3對(duì)BC細(xì)胞增殖和耐藥的功能影響。利用組織微陣列技術(shù)、qRT-PCR和公開數(shù)據(jù)系統(tǒng)評(píng)估DDIT3BC患者預(yù)后的關(guān)系。

結(jié)果:生存分析顯示,在Luminal A BC或接受內(nèi)分泌治療的BC患者中,DDIT3表達(dá)較低的患者預(yù)后較差,且DDIT3表達(dá)與總生存(OS)顯著相關(guān)。MCF-7細(xì)胞中敲低DDIT3后,增殖速率明顯提高,耐藥能力剛好逆轉(zhuǎn)。相反,過表達(dá)DDIT3對(duì)靶細(xì)胞增殖有相對(duì)抑制作用。值得注意的是,抑制DDIT3表達(dá)可上調(diào)TP63,下調(diào)PDGFR,而過表達(dá)DDIT3后的結(jié)果正好相反。

結(jié)論:這些結(jié)果揭示了DDIT3Luminal A BC細(xì)胞增殖和TAM耐藥中起關(guān)鍵作用,并具有潛在的預(yù)后價(jià)值。綜上所述,dddit3可能通過調(diào)節(jié)TP63PDGFR的表達(dá)而在luminal ABC中發(fā)揮作用。

 

該論文中,HEK293T和人乳腺癌(BC)細(xì)胞系MCF-7的體外培養(yǎng)是使用Ausbian特級(jí)胎牛血清完成的。


亚洲va中文字幕无码2020| 国产精品久久久久久吹潮| 国产亚洲精品久久久久久久软件| 免费看大片软件| 99国产精品无码| 老太bbw搡bbbb搡bbbb| 久久久久成人精品免费播放动漫 | 一区二区三区国产精华液区别| 大乳丰满人妻中文字幕日本| 亚洲一区二区观看播放| 欧美bbbwbbwbbwbbw| 鸡毛飞上天电视剧全集观看免费| 国产精品久久久久久久小说 | 日本大尺度床戏做爰3d肉蒲团| 伸进同桌奶罩里摸她胸作文| 乱肉黄蓉合集500篇| 久久人人爽人人片AV人成| 无码国产一区二区三区四区公司| 日本真人做爰免费视频120秒 | 免费国产又色又爽又黄的网站| 欧美性猛交xxxx乱大交3| 久久久性色精品国产免费观看| 精品无码欧美一区二区三区不卡 | 两个人的免费视频| 中文在线资源天堂www| 奇米777 米奇影视狠狠| 贵妇情欲按摩a片| 888影视网| 无码视频一区二区三区| 男主骗女主帮她发育h| 蜜臀久久av无码牛牛影视| 国产麻豆一精品一av一免费| 精品久久久久中文字幕| (原创)露脸自拍[62p]| 久久妇女高潮喷水多长时间| 国产精品天天看天天做夜夜爽| 老熟女高潮喷水了| 学长边洗澡边把我处破了小说| 亚洲日韩一中文字暮| 亚洲国产精品网站在线播放| 成人视频在线观看|